Division of Environmental Physics -
Students Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava
Daniel JAKUBČIN Bc. student
Bc. study 2016 - 2017
Supervisor: Karol Hensel Title: Cold plasma effects on biomolecules Abstract: In this work, we focus on effects of cold non-equilibrium plasma generated by transient spark discharge in atmospheric pressure air on solutions of proteins. Model protein, bovine serum albumin BSA, of three different concentrations (15 μM, 100 μM a 1 mM) and three different solutions (tap water, phosphate buffer, saline) was treated in the cyclic with different plasma exposure time. Our objective was to analyze effects of plasma on the protein by UV-VIS and fluorescence spectroscopy, particularly to evaluate the influence of the input concentration, the type of solution and the plasma exposure time. The results showed effective protein denaturation by the plasma demonstrated by a significant decrease of absorbance and fluorescence of the solution. This protein denaturation by the plasma is not caused by heat, but more likely it is result of reactive species formed by the plasma in solution and change of chemical proportions of solution. Exposure of BSA solution in heavy water and its analysis by FTIR spectroscopy showed a potential for detailed analysis of protein denaturation by analyzing its secondary structure. Besides many interesting results, further experiments are required for better understanding of plasma-protein interaction.