DC transient spark discharge in water: Effects on cells, DNA, proteins and enzymes
Hensel K., Tarabová K., Sano K., Tarabová B., Janda M., Machala Z., Jijie R., Mihai C.T., Gorgan L., Pohoata V., Topala I.
20th Symposium on Applications of Plasma Processes SAPP XX, Tatranská Lomnica (Slovakia), January 17-22, p. USB (2015)
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Abstract: DC-driven transient spark (TS) discharge operated in air in contact with water (phosphate buffered solution (PBS) or deionized water) was used for the treatment of mammalian cells (Vero line normal cells, HeLa line cancerous cells), dsDNA, protein (Bovine Serum Albumin) and enzyme (pepsin). Two systems using different methods of water treatment were used: water electrospray and water electrode. Direct exposure of cells to the discharge and indirect exposure to discharge activated gas flow were compared, as well as the effects of TS discharge and pulsed plasma jet in helium used in parallel experiment. The cells were analyzed for viability and apoptosis. Viability of cells was evaluated by trypan/methylene blue staining, apoptosis by complementary analysis using Annexin V and Propidium iodide, while DNA, protein, and enzyme concentrations were evaluated by UV absorption and fluorescence spectroscopy.
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