Division of Environmental Physics - User: student
Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava

Corona discharges with water electrospray for Escherichia coli biofilm eradication on a surface

Kovaľová Z., Leroy M., Kirkpatrick M., Odic E., Machala Z.
Bioelectrochemistry 112, 91–99 (2016)

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Abstract:

Low-temperature plasma (cold), a new method for the decontamination of surfaces, can be an advantageous alternative to the traditional chemical methods, autoclave or dry heat. Positive and negative corona discharges in air were tested for the eradication of 48-h Escherichia coli biofilms grown on glass slides. The biofilms were treated by cold corona discharge plasma for various exposure times. Water electrospray from the high voltage electrode was applied in some experiments. Thermostatic cultivation of the biofilm, and confocal laser scanning microscopy (CLSM) of the biofilm stained with fluorescent dyes were used for biocidal efficiency quantification. Up to 5 log10 reduction of bacterial concentration in the biofilm was measured by thermostatic cultivation after exposure to both corona discharges for 15min. This decontamination efficiency was significantly enhanced by simultaneous water electrospray through the plasma. CLSM showed that the live/dead ratio after treatment remained almost constant inside the biofilm; only cells on the top layers of the biofilm were affected. DAPI fluorescence showed that biofilm thickness was reduced by about 1/3 upon exposure to the corona discharges with electrospray for 15min. The biofilm biomass loss by about 2/3 was confirmed by crystal violet assay.

Citations:

1.)	Julak J.; Vankova E.; Valkova M; Kasparova P.; Masak J.; Scholtz V.: Combination of non-thermal plasma and subsequent antibiotic treatment for biofilm re-development prevention; FOLIA MICROBIOLOGICA (2020), WoS (2020) -------------
2.)	M. Huang, H. Zhuang, J. Zhao, J. Wang, W. Yan, and J. Zhang: Differences in cellular damages induced by dielectric barrier discharge plasma between Salmonella Typhimurium and Staphylococcus aureus. Bioelectrochemistry 107445 (2019), citation no. X, WoS (2020) -------------
3.)	Z. Xu, X. Zhou, W. Yang, Y. Zhang, Z. Ye, S. Hu, C. Ye, Y. Li, Y. Lan, J. Shen, X. Ye, F. Yang, C. Cheng: In vitro antimicrobial effects and mechanism of air plasma‐activated water on Staphylococcus aureus biofilms, Plasma Process. Polym. X, e1900270 (2020), citation no. 16, WoS (2020) -------------
4.)	R. Múgica-Vidal, E. Sainz-García, A. Álvarez-Ordóñez, M. Prieto, M. González-Raurich, M. López, B. Rojo-Bezares, Y. Sáenz, and F. Alba-Elías: Production of Antibacterial Coatings Through Atmospheric Pressure Plasma: a Promising Alternative for Combatting Biofilms in the Food Industry. Food and Bioprocess Technology 12(8) 1251-1263 (2019), citation no. X, Scopus/INDEX. (2019) -------------
5.)	P. Ranieri, G. McGovern, H. Tse, A. Fulmer, M. Kovalenko, G. Nirenberg, V. Miller, A. Fridman, A. Rabinovich, G. Fridman: Microsecond-Pulsed Dielectric Barrier Discharge Plasma-Treated Mist for Inactivation of Escherichia coli In Vitro, IEEE Trans. Plasma Sci. 47 (1), 395-402 (2019), WoS (2019) -------------
6.)	Kruszelnicki J; Lietz AM; Kushner MJ: Atmospheric pressure plasma activation of water droplets, J. PHYS. D-APPL. PHYS. 52 (35) 355207 (2019), WoS (2019) -------------
7.)	H. Wolfmeier, D. Pletzer, S. C. Mansour, R. E. Hancock: New Perspectives in Biofilm Eradication, ACS Infectious Diseases 4 (2) 93-106 (2018), citation no. 49, WoS/SCOPUS (2018) -------------
8.)	Z. Xu, C. Cheng, J. Shen, Y. Lan, S. Hu, W. Han, & P. K. Chu: In vitro antimicrobial effects and mechanisms of direct current air-liquid discharge plasma on planktonic Staphylococcus aureus and Escherichia coli in liquids. Bioelectrochemistry, 121, 125-134 (2018), citation no. X, INDEX (2018) -------------
9.)	A. Kuzminova, T. Kretková, O. Kylián, J. Hanuš, I. Khalakhan, V. Prukner, E. Doležalová, M. Šimek and H. Biederman: Etching of polymers, proteins and bacterial spores by atmospheric pressure DBD plasma in air. Journal of Physics D: Applied Physics 50(13), 135201 (2017), citation no. X, WoS+SCOPUS (2017) -------------
10.)	B. A. Niemira: Targeting Biofilms with Cold Plasma: New Approaches to a Persistent Problem. Biofilms (2017) (2017) -------------
11.)	C. Smet, M. Baka, A. Dickenson, J.L. Walsh, V.P. Valdramidis, and J.F. Van Impe: Antimicrobial efficacy of cold atmospheric plasma for different intrinsic and extrinsic parameters. Plasma Processes and Polymers 15(2) 93-106 (2017), citation no. X, Scopus/INDEX. (2017) -------------
12.)	B. Sun, Y. Xin, X. Zhu, Z. Gao, Z. Yan, and T. Ohshima: Effects of shock waves, ultraviolet light, and electric fields from pulsed discharges in water on inactivation of Escherichia coli. Bioelectrochemistry 120, 112-119 (2017), citation no. X, Scopus/INDEX. (2017) -------------
13.)	J. Zabielska, A. Tyfa, and A. Kunicka-Styczyńska: Methods for eradication of the biofilms formed by opportunistic pathogens using novel techniques–A review. Folia Biologica et Oecologica 12.1, 26-37 (2016) (2016) -------------