Division of Environmental Physics - User: igor
Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava

DC corona discharge for bacterial biofilm decontamination

Kovaľová Z., Leroy M., Machala Z., Odic E.
15th High Pressure Low Temperature Plasma Chemistry Symposium (HAKONE XV), Brno (Czech republic), September 11-16, p. 424-427 (2016)

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Abstract:

DC corona discharges in air were tested for the eradication of 48-hour Escherichia coli biofilms on glass slides. Thermostatic cultivation and confocal laser scanning microscopy of the biofilm stained with fluorescent dyes were used for biocidal efficiency quantification. Up to 5.7 log10 reduction of bacterial concentration in the biofilm was measured after exposure to DC corona discharges for 15 min. CLSM showed that live/dead ratio in the biofilm was decreasing after plasma treatment and that the biofilm thickness was reduced for both corona polarities. The
biofilm biomass loss was supported by crystal violet assay.