Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava |
Application of miniature glidarc to rejection of bacterial contamination from the teflon surfaceKrupski P., Kwiatkowski M., Terebun P., Diatczyk J., Stryczewska H.D., Pawłat J., Machala Z., Hensel K. |
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Abstract: Plasma activated water (PAW) is a source of various chemical species useful for the plant growth, development, and stress response. In the present study the PAW was generated by a transient spark discharge (TS) operated in ambient air and used on maize corns and seedlings in the 3-day paper rolls cultivation followed by 10-day hydroponics cultivation. For 3-day cultivation two pre-treatments were established, “'priming PAW” and “rolls PAW”, with corns imbibed for 6-hours in the PAW, and then daily watered by fresh water and PAW, respectively. The roots and the shoot were then analysed for guaiacol peroxidase (G-POX, POX) activity, roots tissues for their lignification and root cell walls for in situ POX activity. To evaluate the potential of PAW in alleviation abiotic stress, ten randomly selected seedlings were hydroponically cultivated for the following 10-days in 0.5 Hoagland nutrient solutions with and without 150 uM As. The seedlings were then analysed for POX and catalase (CAT) activities after As treatment, their leaves for photosynthetic pigments concentration, and leaves and roots for As concentration. The PAW improved the growth of the 3-days-old seedlings in terms of the root and the shoot length, while roots revealed accelerated endodermal development. After the following 10-day cultivation, roots from PAW pre-treatment were shorter, thinner, but more branched than the control roots. The PAW also enhanced the POX activity immediately after the imbibition and in the 3-days-old roots. After 10-day hydroponic cultivation, antioxidant response depended on the PAW pre-treatment. CAT activity was higher in As treatments compared to the corresponding PAW treatments, while POX activity was not obvious, and its elevated activity was found only in the priming PAW treatment. The PAW pre-treatment protected chlorophylls in the following treatments combined with As, while carotenoids increased in treatments despite PAW pre-treatment. Finally, the ac-cumulation of As in the roots was not affected by PAW pre-treatment but increased in the leaves.
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