Division of Environmental Physics - User: veronika
Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava

Dynamics of Singlet Oxygen-Triggered, RONS-Based Apoptosis Induction after Treatment of Tumor Cells with Cold Atmospheric Plasma

Bauer G., Sersenová D., Graves D. B. & Machala Z.
Sci. Reports 9 (13931), 1-34 (2019)

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Abstract:

Treatment of tumor cells with cold atmospheric plasma (CAP) or with plasma-activated medium (PAM) leads to a biochemical imprint on these cells. This imprint is mediated by primary singlet oxygen, which is mainly generated through the interaction between CAP-derived H2O2 and NO2−. This imprint is induced with a low efficiency as local inactivation of a few membrane-associated catalase molecules. As sustained generation of secondary singlet oxygen by the tumor cells is activated at the site of the imprint, a rapid bystander effect-like spreading of secondary singlet oxygen generation and catalase inactivation within the cell population is thus induced. This highly dynamic process is essentially driven by NOX1 and NOS of the tumor cells, and finally leads to intercellular RONS-driven apoptosis induction. This dynamic process can be studied by kinetic analysis, combined with the use of specific inhibitors at defined time intervals. Alternatively, it can be demonstrated and quantified by transfer experiments, where pretreated cells are mixed with untreated cells and bystander signaling is determined. These studies allow to conclude that the specific response of tumor cells to generate secondary singlet oxygen is the essential motor for their self-destruction, after a singlet oxygen-mediated triggering process by CAP or PAM.

Citations:

1.)	Noori H; Raud J; Talviste R; Jogi I; Water Dissolution of Nitrogen Oxides Produced by Ozone Oxidation of Nitric Oxide; Ozone-Sci. Eng. xxx (2021) WoS (2021) -------------
2.)	Ma MY; Cheng H; Sun FS; Lu XP; He GY; Laroussi M: Differences in Cytotoxicity Induced by Cold Atmospheric Plasma and Exogenous RONS Solutions on Human Keratinocytes and Melanoma Cells; IEEE TRANS. RADIATION AND PLASMA MEDICAL SCIENCES 5 (6) 835-842 (2021) WoS (2021) -------------
3.)	Yan D.; Horkowitz A.; Wang Q. et al.: On the selective killing of cold atmospheric plasma cancer treatment: Status and beyond; PLASMA PROCESSES AND POLYMERS 18 (10) e2100020 (2021) WoS (2021) -------------
4.)	Rasouli M.; Mehdian H.; Hajisharifi K. et al.: Plasma-activated medium induces apoptosis in chemotherapy-resistant ovarian cancer cells: High selectivity and synergy with carboplatin PLASMA PROCESSES POLYM. 18 (9) e2100074 (2021) WoS (2021) -------------
5.)	Semmler M.L.; Bekeschus S.; Schaefer M.; et al.: Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment; CANCERS 12 (2) 269, WoS (2020) -------------
6.)	E. Sardella: Generation and Control of Plasma Induced Reactive Oxygen/Nitrogen Species in Liquids: a Route Toward Selective Cell Responses; 8th International Conference on Plasma Medicine, Seoul, Korea (2020) (2020) -------------
7.)	Bengtson C; Bogaerts A: On the Anti-Cancer Effect of Cold Atmospheric Plasma and the Possible Role of Catalase-Dependent Apoptotic Pathways; Cells 9(10) 2330 (2020) WoS (2020) -------------
8.)	Dai XF; Bazaka K; Thompson EW; Ostrikov K; Cold Atmospheric Plasma: A Promising Controller of Cancer Cell States; Cancers 12(11) 3360 (2020) WoS (2020) -------------
9.)	Malyavko A.; Yan DY; Wang QH; Klein AL; Patel KC; Sherman JH; Keidar M: Cold atmospheric plasma cancer treatment, direct versus indirect approaches; MATERIALS ADVANCES 1 (6) 1494-1505 (2021) WoS (2020) -------------
10.)	Privat-Maldonado A., Bengtson C., Razzokov J., Smits E., Bogaerts A.: Modifying the tumour microenvironment: Challenges and future perspectives for anticancer plasma treatments. Cancers 11 (12) (2019) 1920, SCI; SCOPUS (2019) -------------