Cold air plasma of streamer discharge or plasma activated water for antibacterial effects and wound healing
Machala Z., Suchoňová M., Konečná B., Tarabová B., Pásztor S., Tóthová Ľ.
International Meeting on Plasma Cosmetic Science, Orléans (FR), Nov 25-27, p. 20 (2019)
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Abstract: Antibacterial effects of cold atmospheric plasmas or plasma activated water (PAW) are generally accepted as demonstrated by many researchers. We present antibacterial effects of cold plasma generated by streamer discharge in ambient air using a portable plasma pen in vitro on agar plates with E. coli and bacteria from human hand skin using scratches mimicking cutting wounds. Longer plasma treatment results in wider decontaminated scratch areas but the age of pre-cultivated bacteria is an important parameter: the antibacterial effect is weakened when bacteria are pre-grown on agar for more than 6 h. This plasma discharge was also preliminarily tested in a case study of large surface wound (~20 cm2) on volunteer’s hand. The plasma treatment repeated once a day for 7 days and combined only with rinsing by tap water resulted in very little infection spread, very limited inflammation and a fast recovery of the skin tissue within 15 days.
PAW produced by transient spark air discharge with water electrospray also demonstrated significant antibacterial effects if applied within a few minutes post activation. For longer conservation, such PAW can be quickly frozen post plasma activation, stored at -20 C and defrost just before application. The PAW inhibitory effect of bacterial growth is dependent on the bacteria concentration: especially efficient for the range of 10^3-10^7 CFU/ml.
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