The effects of plasma-activated phosphate buffered saline on monolayers and spheroids of cancer cells
Kužmová D., Machala Z., Gbelcová H.
9th Central European Symposium on Plasma Chemistry CESPC-9 + COST Action CA19110 Plasma Applications for Smart and Sustainable Agriculture (PlAgri), Vysoké Tatry (Slovakia), September 4-9, p. 72 (2022)
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Abstrakt: Conventional cancer therapies have a high level of toxicity for human body and often burden patients with severe side effect. In addition, in many cases the tumour becomes resistant to chemotherapeutic agents. Therefore, it is necessary to search for new strategies to cure cancer. One of the promising strategies is found in plasma medicine. Cold plasma has a potential to prolong patient's life and make it more valuable if the previous conventional cancer therapies fail. The main role in plasma therapy is played by reactive oxygen and nitrogen species (RONS) generated in the plasma and plasma-activated liquids (PAL) [1].
The aim of this study is to investigate the effect of indirect application of cold plasma, via plasma activated phosphate buffered saline (PBS), to drug sensitive human breast adenocarcinoma cell lines MCF-7 (kindly provided by Prof. Ján Kovář, Charles University, Prague) and the Paclitaxel-resistant sub-lines MCF-7/PAX [2,3]. MCF-7 and MCF-7/PAX were incubated in PBS treated by cold atmospheric plasma of streamer corona discharge.
We tested the effect of plasma-activated PBS (PAPBS) treated for 5 and 10 minutes added to 2D cell monolayer and 3D tumour models – spheroids. The concentration of RONS in PAPBS linearly increased with the plasma activation time. [4]. After 30 minutes of cell incubation in PAPBS, we observed the viability reduction of both cell lines. Metabolic WST-1 assay has shown a decrease of the number of viable cells depending on the plasma treatment time. The following microscopic analysis proved, that PAPBS induced processes leading to cell death in both sensitive cancer cell line and the resistant sub-line of human breast tumor cells. PAPBS also seems to inhibit the growth and induce a disintegration of spheroids.
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