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Paulína HALUŠKOVÁ alumni student
MSc. study 2017 - 2019
Supervisor: Zdenko Machala Consultant: Julia Bandow Title: Effects of cold plasma to heme-dependent enzymes Abstract: This work focuses on long-term goal to gain an in-depth
understanding of the mechanisms of interaction between proteins
and different gas plasmas needed for plasma biomedical
applications. The objectives of this work are to compare the
effects of two air plasma sources - dielectric barrier discharge
(DBD) and transient spark (TS) on heme-dependent enzymes
KatE, KatG, EfeB and Scdyp2. Enzymes were purified by
immobilized metal ion affinity chromatography and treated with
DBD up to 10 min or up to 5 min with TS. After the plasma
treatment, concentration assay, activity assay and UV-VIS
spectroscopy were realized for evaluation of the differences of the
effects between the enzymes and between different plasma
discharges.
Results showed that KatE is the most resistant enzyme, because
after 5 min of plasma treatment with DBD it was fully active and
after 10 min of the treatment with the same discharge it still had
70% of the remaining activity. Enzyme Scdyp2 was inactivated
in the shortest time. After 5 min of treatment with DBD it had
only 13% of remaining activity and 10 min of plasma treatment
caused its total inactivation. The energy consumption of TS per 1
ml of a treated sample was 1.25 times higher than for DBD,
therefore the effect of TS on these enzymes was slightly stronger.
Besides many interesting results, for better understanding of the
effect of cold atmospheric plasma on proteins, structural analysis
of the treated enzymes is needed.
Bc. study 2016 - 2017
Supervisor: Zdenko Machala Consultant: Katarína Sobolíková Title: Testing of the effects cold plasma and plasma activated water with parodontitis therapy Abstract:
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Active
PhD students:
Jana KŠANOVÁ
Darina KUŽMOVÁ
Ramin MEHRABIFARD
Zuzana OKRUHLICOVÁ
Pankaj PAREEK
Gokul SELVARAJ
Sergei SMIRNOV
Peter TÓTH
MSc/Bc students:
Dominik DIŇA
Alumni
PhD students
MSc students
Bc students
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