Division of Environmental Physics -
Students Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava
Slavomir PÁSZTOR Bc. student
Bc. study 2017 - 2019
Supervisor: Zdenko Machala Consultant: -- Title: Testing plasma activated water for the therapy of urinary tract infections Abstract: In this bachelor thesis we tested the effects of plasma activated water (PAW) in the treatment of urinary tract infections. We compared the effects of PAW with control saline solution (PHYS 0,15 M NaCl PBS-Phosphate Buffered Saline). We observed the inhibitory effect of PAW on bacterial proliferation at different concentrations of bacteria and at different ratios between PAW/PHYS and the bacteria in liquid nutrient medium. These experiments were performed on uropatogenic strain of Escherichia coli (UTI). Testing was done in two models of experiments. The first model was in vitro testing, which was performed in 96-well microtiter plates, in which the absorbance of the samples corresponding to the number of bacteria was measured spectrophotometrically. The second model was in vivo testing, performed on female mice. We infected mice and then applied PAW/PHYS. After a specified time, we removed the bladder and kidneys from mice, which we subsequently homogenized and sown the homogenate on Petri dishes with nutrient medium, in which we counted the grown colonies after the incubation time. We quantitatively evaluated the effect of PAW in living organisms.
The results of in vitro experiments show that PAW is the most effective disinfectant when it is found in a sample in volume predominance. At a 1:9 ratio (UTI:PAW), when PAW accounted for 90% of the sample volume, we achieved the best results. This means that the number of bacteria in the samples with PAW, compared to the samples with PHYS, did not increase over time or that the difference between the two samples was significant after eight-hour incubation, at which the values for PAW were lower.
The results of the in vivo experiment demonstrated that PAW had an effect in inhibiting bacterial proliferation in mouse bladder, as can be seen from the logarithmic reduction results for PAW and PHYS groups and difference between them which was 1 log. Based on this, we assume that PAW has prevented the development of the kidney infection and by that we have met one of the aims of this work.