Division of Environmental Physics -
Students Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava
Lucia PAKOSOVÁ alumni student
MSc. study Supervisor: Zdenko Machala Consultant: -- Title: Investigations of the effects of reactive plasma species to cells using genetic mutants of yeasts Abstract:
Bc. study 2012 - 2013
Supervisor: Zdenko Machala Consultant: -- Title:
Abstract: The aim of this work is to focus on the mechanisms of action of non-equilibrium plasma generated transient spark discharges in air at atmospheric pressure on eukaryotic cells in order to better understand interactions between cold plasma and a live cell.
We have worked with Saccharomyces cerevisiae yeast cells, we compared the effect of transient sparks positive polarity on cell survival in standard and mutant strain, which was present in its genome gene mutation has played an important role in the cell cycle and cell antioxidant protective mechanisms. Equally important was to compare two methods of working non-equilibrium plasma cells through direct and indirect method of monitoring the impact of time on the effect of plasma treatment.
The measurements were evaluated mainly by % of survival of plasma treated cells relative to the reference (untreated) cells. The results of the comparison of direct and indirect confirmed working more efficiency to reduce cell viability by direct discharge passage, which was confirmed by a significant effect of time on the inactivation of plasma effect on cells.
The results of working with mutant strains brought new assumptions about the possible role of some active plasma particles. Proved to be very effective plasma treatment of some mutant strains, in particular strains BY4742Δsod1, 2, in which the cells lack the enzyme superoxide dismutase protecting their effect on superoxide anion O2-.