Division of Environmental Physics -
Students Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava
Dominika MIHÁLIKOVÁ alumni student
MSc. study 2018 - 2020
Supervisor: Zdenko Machala Consultant: Helena Gbelcová Title: Adaptation of the portable source of cold plasma for pulsing regime and testing of its effects on cancerous and healthy epithelial cells Abstract:
Bc. study 2017 - 2018
Supervisor: Zdenko Machala Consultant: Helena Gbelcová Title: Effects of plasma activated medium on cancerous and healthy cells Abstract: The main objective of this bachelor thesis was to experimentally test the effects of plasma activated medium (PAM) on non-cancer and cancer cells in vitro. We worked with human epithelial melanoma cells – A375 and human embryonic epithelial kidney cells – HEK293T and we used two types of cold atmospheric plasma discharges – streamer corona (SC) and transient spark (TS) with two different exposure times (0.5 min/ml and 2 min/ml) to activate the medium. We applied the PAM on cells 2 hours after its exposure to the plasma. We investigated the effects of PAM on cells by metabolic MTT assay and we studied microscopic changes of confluence, size and shape of cells using optical microscopy. We measured the specific absorbance of our samples, which we then used to calculate viability of cells. The measurements were done 24 hours and 72 hours after the application of PAM on cells.