Division of Environmental Physics -
Students Faculty of Mathematics, Physics and Informatics, Comenius University Bratislava
Martina VELÍSKOVÁ alumni student
Bc. study 2017 - 2018
Supervisor: Zdenko Machala Consultant: Július Hodossy Title: Testing of the possibilities of plasma activated water in wound healing Abstract: The main objective of this work was to study lethal or inhibiting effects of water activated by cold plasma of spark discharge on bacteria and the possibility to use it for wound disinfection. We used a spectrophotometric methods to measure changes in absorbance of various dilutions in 96-well plates in time. The wells contained skin bacteria and we compared the effects of plasma activated water (PAW) and physiological solution which is nowadays used for disinfection of surface wounds. The increasing absorbance indicated the increasing concentration of bacteria in dilution, which indicates that the bacteria were multiplying. For quantitative evaluation we used Petri dishes with agar to count colonies that grew on agar after PAW or physiological solution affected the bacteria.
Results of experiments of absorbance measurements show that PAW is more effective in inhibition of bacterial reproduction when it affects not so much diluted bacteria for 10 minutes. The difference between PAW and physiological solution is observable after shorter period of time (two hours) with 10 minutes influence, while after affecting bacteria for 30 minutes or after affecting more diluted bacteria the inhibition effects are observable after 24 hours.
Results of experiments with Petri dishes show that PAW is more efficient in inactivating skin bacteria (compared to physiological solution) when affecting bacteria for shorter times – after 10 minutes PAW is 5 – 10% more effective than physiological solution. However, the largest logarithmic reduction was observed after 30 minutes of PAW affecting bacteria compared to physiological solution. If bacteria are diluted enough, the logarithmic reduction of PAW (3,22) was much stronger than the logarithmic reduction in physiological solution (1,75).