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Dreninová, V.
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Halušková, P.
Hassan, M.
Hennecke, A.
Chládeková, L.
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Valovič, P.
Velísková, M.
Záňová, V.
Zigo, J.
Žilková, A.
								

								

								Lenka Chládeková
MSc. level: 2007 - 2009
Supervisor: Zdenko Machala
Title (en): 

Title (sk): 
Mechanizmy interakcie plazmy s bunkou
Abstract (en):  hide
In the graduation thesis we experimentally investigated biological effects of
atmospheric pressure plasma of positive and negative corona and spark discharge
generated in ambient air on bacterial cell Salmonella typhimurium and the role of
inactivation agents in this process. Biodecontamination effect of discharges on
microorganisms is documented by a decrease of the number of surviving cells after
exposure and by the parameter of efficiency. The extent of oxidation damage in
microorganisms is characterized by an increase in formation of secondary lipid
peroxidation products in the membranes of exposed cells via reaction with
thiobarbituric acid. We discovered a significant increase in oxidation damage of
membrane lipids in cells exposed to the discharges with respect to the control, which
demonstrates an important role of radicals in the inactivation process. The experimental
results related to the separation of the ultraviolet radiation generated in the plasma refer
to its minor role in the used electrical discharges.
Abstract (sk)