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								Abedivaraki, M.
Bartková, D.
Buchholcerová, H.
Cíbiková, M.
Cimerman, R.
Damek, M.
Dreninová, V.
Dvonč, L.
Gálik, J.
Giertl, D.
Halušková, P.
Hassan, M.
Hennecke, A.
Chládeková, L.
Ivanová, P.
Jakubčin, D.
Janíková, K.
Jankovič, R.
Jedlovský, I.
Kintler, M.
Kováč, M.
Kovaľová, Z.
Kučerová, K.
Kukura, S.
Kunecová, D.
Lavrikova, A.
Leštinská, L.
Leštinský, M.
Maslík, J.
Maťáš, E.
Menthéour , R.
Miháliková, D.
Mišenko, P.
Molnár, M.
Ndiffo Yemeli, G.
Niklová, A.
Okruhlicová, V.
Omasta, S.
Pakosová, L.
Pásztor, S.
Pavle, M.
Pelach, M.
Polakovič, A.
Poljak, M.
Pongrác, B.
Pračko, M.
Račková, D.
Roubíček, J.
Saedi , M.
Selvek, M.
Sersenová, D.
Seyfi, P.
Sič, J.
Strižencová, L.
Sučanská, A.
Štípala, P.
Tarabová, B.
Valovič, P.
Velísková, M.
Záňová, V.
Zigo, J.
Žilková, A.
								

								

								Katarína Kučerová
Bc. level: 2011 - 2012
Supervisor: Karol Hensel
Title (en): 
Biodecontamination by pulsed electrical discharges
Title (sk): 
Biodekontaminácia  impulznými elektrickými výbojmi
Abstract (en)
Abstract (sk)
MSc. level: 2012 - 2014
Supervisor: Karol Hensel
Title (en): 
Electrical discharge in contact with aqueous medium, their biological and chemical effects
Title (sk): 
Elektrické výboje v kontake s vodnými roztokmi, ich biologické a chemické účinky
Abstract (en):  hide
We investigate chemical and biological effects of the transient spark discharge, generated by DC high voltage of positive polarity in atmospheric pressure air and applied to water solutions. We used water electrode system, where the liquid repetitively flow through the discharge zone. We analyzed changes induced by the discharge in two main solutions (sodium dihydrogen phosphate NaH2PO4 and saline solution NaCl) and their buffered counterparts (phosphate buffer and phosphate saline buffer). To explore biological effects of the discharge, Bacillus cereus, Escherichia coli and cancerous HeLa cells were used as model organisms. We monitored pH, conductivity and temperature of plasma treated solutions. We measured concentration of hydrogen peroxide H2O2 through its reaction with titanyl ions of titanium sulfate and formation of colored complex of pertitanic acid. By using indigo colorimetric method we evaluated the concentration of ozone O3 and by the colorimetric assay kit we measured the concentrations of nitrites NO2- and nitrates NO3-. Peroxynitrite ONOO concentration was determined by fluorescence probe DCF-DA. All the parameters were investigated in dependence on plasma treatment time. pH decrease, conductivity and temperature increase as well as the increase of concentration of all active species in time was observed. On the other hand, all these parameters were found independent of the flow rate of solution through the system. After the discharge we observed a decrease in concentrations of hydrogen peroxide, ozone, nitrites and the increase of nitrates. We refer to low selectivity of the indigo method toward ozone, and possibility of indigo solution being decolorized by hydroxyl radical, too. In the saline solution after 10 minutes of plasma treatment we achieved the decrease of bacterial population by 1.2 log reduction with B. cereus, 3 log reduction with E. coli and we inactivated 49 % of HeLa cells population. Finally, the effects induced by the transient spark in water electrode system were compared with the results achieved in the system of water electro-spray.
Abstract (sk)
PhD. level: 2014 - 2021
Supervisor: Karol Hensel
Title (en): 
Cold plasma in a contact with liquid - Its chemical effects and selected biomedical and agriculture applications
Title (sk): 
Studená plazma v kontakte s kvapalinami - Jej chemické účinky a vybrané biomedicínske a poľnohospodárske aplikácie
Abstract (en)
Abstract (sk)